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Science 8 December 2000:
Vol. 290. no. 5498, pp. 1975 - 1978
DOI: 10.1126/science.290.5498.1975
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Reports

Multigenerational Cortical Inheritance of the Rax2 Protein in Orienting Polarity and Division in Yeast

Tracy Chen,1 Takatoshi Hiroko,2 Amitabha Chaudhuri,1 Fumika Inose,2 Matthew Lord,1 Shigeko Tanaka,2 John Chant,1* Atsushi Fujita2*

Diploid yeast cells repeatedly polarize and bud from their poles, probably because of highly stable marks of unknown composition. Here, Rax2, a membrane protein, was shown to behave as such a mark. The Rax2 protein itself was inherited immutably at the cell cortex for multiple generations, and Rax2 was shown to have a half-life exceeding several generations. The persistent inheritance of cortical protein markers would provide a means to couple a cell's history to the future development of a precise morphogenetic form.

1 Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.
2 National Institute of Bioscience & Human Technology, AIST 1-1 Higashi, Tsukuba 305-8566, Japan.
*   To whom correspondence should be addressed. E-mail: chant{at}fas.harvard.edu (J.C.) and atsushi{at}nibh.go.jp (A.F.).

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