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Published Online December 8, 2005
Science DOI: 10.1126/science.1116804

Reports

Submitted on June 30, 2005
Accepted on November 21, 2005

Herpesviral Protein Networks and Their Interaction with the Human Proteome

Peter Uetz 1, Yu-An Dong 2, Christine Zeretzke 3, Christine Atzler 3, Armin Baiker 4, Bonnie Berger 2, Seesandra Rajagopala 1, Maria Roupelieva 4, Dietlind Rose 3, Even Fossum 3, Jürgen Haas 3*

1 Institut für Genetik, Forschungszentrum Karlsruhe, Postfach 3640, Karlsruhe, D-76021 Germany.
2 Department of Mathematics, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.
3 Max-von-Pettenkofer Institut, Ludwig-Maximilians-Universität München, Pettenkoferstrasse 9a, 80336 München, Germany.
4 Max-von-Pettenkofer Institut, Ludwig-Maximilians-Universität München, Pettenkoferstrasse 9a, 80336 Mnchen, Germany.

* To whom correspondence should be addressed.
Jürgen Haas , E-mail: haas{at}lmb.uni-muenchen.de

The comprehensive yeast-two-hybrid analysis of intraviral protein interactions in two members of the herpesvirus family, Kaposi Sarcoma associated Herpesvirus (KSHV) and Varicella Zoster Virus (VZV), revealed 123 and 173 interactions, respectively. Viral protein interaction networks resemble single, highly coupled modules, whereas cellular networks are organized in separate functional submodules. Predicted and experimentally verified interactions between KSHV and human proteins were used to connect the viral interactome into a prototypical human interactome and to simulate infection. The analysis of the combined system showed that the viral network adopts cellular network features, and that protein networks of herpesviruses and possibly other intracellular pathogens have distinguishing topologies.


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