Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 15 December 2000:
Vol. 290. no. 5499, pp. 2148 - 2152
DOI: 10.1126/science.290.5499.2148
Prev | Table of Contents | Next

Reports

The Bacterial Flagellar Cap as the Rotary Promoter of Flagellin Self-Assembly

Koji Yonekura,1* Saori Maki,1* David Gene Morgan,2 David J. DeRosier,3 Ferenc Vonderviszt,4 Katsumi Imada,1 Keiichi Namba15dagger

The growth of the bacterial flagellar filament occurs at its distal end by self-assembly of flagellin transported from the cytoplasm through the narrow central channel. The cap at the growing end is essential for its growth, remaining stably attached while permitting the flagellin insertion. In order to understand the assembly mechanism, we used electron microscopy to study the structures of the cap-filament complex and isolated cap dimer. Five leg-like anchor domains of the pentameric cap flexibly adjusted their conformations to keep just one flagellin binding site open, indicating a cap rotation mechanism to promote the flagellin self-assembly. This represents one of the most dynamic movements in protein structures.

1 Protonic NanoMachine Project, ERATO, JST, 3-4 Hikaridai, Seika, Kyoto 619-0237, Japan.
2 Department of Biophysics, Boston University School of Medicine, Boston, MA 02118, and Department of Cell Biology, Harvard Medical School, Boston, MA 02254, USA.
3 Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02254, USA.
4 Department of Physics, University of Veszprém, Egyetem Street 10, H-8201 Veszprém, Hungary.
5 Advanced Technology Research Laboratories, Matsushita Electric Industrial Co., Ltd., 3-4 Hikaridai, Seika, Kyoto 619-0237 Japan.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: keiichi{at}crl.mei.co.jp

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Flagella of an Atypical Enteropathogenic Escherichia coli Strain Are Required for Efficient Interaction with and Stimulation of Interleukin-8 Production by Enterocytes In Vitro.
S. C. F. Sampaio, T. A. T. Gomes, C. Pichon, L. du Merle, S. Guadagnini, C. M. Abe, J. L. M. Sampaio, and C. Le Bouguenec (2009)
Infect. Immun. 77, 4406-4413
   Abstract »    Full Text »    PDF »
FliZ Is a Posttranslational Activator of FlhD4C2-Dependent Flagellar Gene Expression.
S. Saini, J. D. Brown, P. D. Aldridge, and C. V. Rao (2008)
J. Bacteriol. 190, 4979-4988
   Abstract »    Full Text »    PDF »
What's the point of the type III secretion system needle?.
A. J. Blocker, J. E. Deane, A. K. J. Veenendaal, P. Roversi, J. L. Hodgkinson, S. Johnson, and S. M. Lea (2008)
PNAS 105, 6507-6513
   Abstract »    Full Text »    PDF »
Flagella of Pyrococcus furiosus: Multifunctional Organelles, Made for Swimming, Adhesion to Various Surfaces, and Cell-Cell Contacts..
D. J. Nather, R. Rachel, G. Wanner, and R. Wirth (2006)
J. Bacteriol. 188, 6915-6923
   Abstract »    Full Text »    PDF »
{sigma}28-Dependent Transcription in Salmonella enterica Is Independent of Flagellar Shearing..
V. Rosu and K. T. Hughes (2006)
J. Bacteriol. 188, 5196-5203
   Abstract »    Full Text »    PDF »
Transcriptional and Translational Control of the Salmonella fliC Gene..
P. Aldridge, J. Gnerer, J. E. Karlinsey, and K. T. Hughes (2006)
J. Bacteriol. 188, 4487-4496
   Abstract »    Full Text »    PDF »
Simultaneous Display of Multiple Foreign Peptides in the FliD Capping and FliC Filament Proteins of the Escherichia coli Flagellum.
K. Majander, T. K. Korhonen, and B. Westerlund-Wikstrom (2005)
Appl. Envir. Microbiol. 71, 4263-4268
   Abstract »    Full Text »    PDF »
The Type III-Dependent Hrp Pilus Is Required for Productive Interaction of Xanthomonas campestris pv. vesicatoria with Pepper Host Plants.
E. Weber, T. Ojanen-Reuhs, E. Huguet, G. Hause, M. Romantschuk, T. K. Korhonen, U. Bonas, and R. Koebnik (2005)
J. Bacteriol. 187, 2458-2468
   Abstract »    Full Text »    PDF »
A partial atomic structure for the flagellar hook of Salmonella typhimurium.
T. R. Shaikh, D. R. Thomas, J. Z. Chen, F. A. Samatey, H. Matsunami, K. Imada, K. Namba, and D. J. DeRosier (2005)
PNAS 102, 1023-1028
   Abstract »    Full Text »    PDF »
Docking of cytosolic chaperone-substrate complexes at the membrane ATPase during flagellar type III protein export.
J. Thomas, G. P. Stafford, and C. Hughes (2004)
PNAS 101, 3945-3950
   Abstract »    Full Text »    PDF »
Domain movements of HAP2 in the cap-filament complex formation and growth process of the bacterial flagellum.
S. Maki-Yonekura, K. Yonekura, and K. Namba (2003)
PNAS 100, 15528-15533
   Abstract »    Full Text »    PDF »
Type III secretion systems and bacterial flagella: Insights into their function from structural similarities.
A. Blocker, K. Komoriya, and S.-I. Aizawa (2003)
PNAS 100, 3027-3030
   Abstract »    Full Text »    PDF »
Prokaryotic motility structures.
S. L. Bardy, S. Y. M. Ng, and K. F. Jarrell (2003)
Microbiology 149, 295-304
   Abstract »    Full Text »    PDF »
Bright Lights, Abundant Operons--Fluorescence and Genomic Technologies Advance Studies of Bacterial Locomotion and Signal Transduction: Review of the BLAST Meeting, Cuernavaca, Mexico, 14 to 19 January 2001.
R. B. Bourret, N. W. Charon, A. M. Stock, and A. H. West (2002)
J. Bacteriol. 184, 1-17
   Full Text »    PDF »
Salmonella Flagellin-Dependent Proinflammatory Responses Are Localized to the Conserved Amino and Carboxyl Regions of the Protein.
T. D. Eaves-Pyles, H. R. Wong, K. Odoms, and R. B. Pyles (2001)
J. Immunol. 167, 7009-7016
   Abstract »    Full Text »    PDF »
Polar Flagellar Motility of the Vibrionaceae.
L. L. McCarter (2001)
Microbiol. Mol. Biol. Rev. 65, 445-462
   Abstract »    Full Text »    PDF »
Protein secretion and the pathogenesis of bacterial infections.
V. T. Lee and O. Schneewind (2001)
Genes & Dev. 15, 1725-1752
   Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)