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Science 22 June 2001:
Vol. 292. no. 5525, pp. 2333 - 2337
DOI: 10.1126/science.1060214
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Reports

Recruitment of HAT Complexes by Direct Activator Interactions with the ATM-Related Tra1 Subunit

Christine E. Brown,12 LeAnn Howe,12 Kyle Sousa,12 Stephen C. Alley,3 Michael J. Carrozza,12 Song Tan,2 Jerry L. Workman1*

Promoter-specific recruitment of histone acetyltransferase activity is often critical for transcriptional activation. We present a detailed study of the interaction between the histone acetyltransferase complexes SAGA and NuA4, and transcription activators. We demonstrate by affinity chromatography and photo-cross-linking label transfer that acidic activators directly interact with Tra1p, a shared subunit of SAGA and NuA4. Mutations within the COOH-terminus of Tra1p disrupted its interaction with activators and resulted in gene-specific transcriptional defects that correlated with lowered promoter-specific histone acetylation. These data demonstrate that the essential Tra1 protein serves as a common target for activators in both SAGA and NuA4 acetyltransferases.

1 Howard Hughes Medical Institute,
2 Department of Biochemistry and Molecular Biology,
3 Department of Chemistry, The Pennsylvania State University, 306 Althouse Laboratory, University Park, PA 16802, USA.
*   To whom correspondence should be addressed: E-mail: jlw10{at}psu.edu

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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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