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Originally published in Science Express on 8 December 2005
Science 13 January 2006:
Vol. 311. no. 5758, pp. 239 - 242
DOI: 10.1126/science.1116804
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Reports

Herpesviral Protein Networks and Their Interaction with the Human Proteome

Peter Uetz,1* Yu-An Dong,2* Christine Zeretzke,3 Christine Atzler,3 Armin Baiker,3 Bonnie Berger,2 Seesandra V. Rajagopala,1 Maria Roupelieva,3 Dietlind Rose,3 Even Fossum,3 Jürgen Haas3{delta}

The comprehensive yeast two-hybrid analysis of intraviral protein interactions in two members of the herpesvirus family, Kaposi sarcoma–associated herpesvirus (KSHV) and varicella-zoster virus (VZV), revealed 123 and 173 interactions, respectively. Viral protein interaction networks resemble single, highly coupled modules, whereas cellular networks are organized in separate functional submodules. Predicted and experimentally verified interactions between KSHV and human proteins were used to connect the viral interactome into a prototypical human interactome and to simulate infection. The analysis of the combined system showed that the viral network adopts cellular network features and that protein networks of herpesviruses and possibly other intracellular pathogens have distinguishing topologies.

1 Institut für Genetik, Forschungszentrum Karlsruhe, Postfach 3640, Karlsruhe, D-76021 Germany.
2 Department of Mathematics, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.
3 Max-von-Pettenkofer Institut, Ludwig-Maximilians-Universität München, Pettenkofer-strasse 9a, 80336 München, Germany.

* These authors contributed equally to this work.

{delta} To whom correspondence should be addressed. E-mail: haas{at}lmb.uni-muenchen.de

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